CIRAD | TropGeneDB | Oryza Tag Line | GreenPhylDB | Genoplante
Home >> Tools

Keywords Search

You could select a set of locus with a keyword search throught features annotations. Moreover, all the linked FST inside or in the promoter region of the selected genes are displayed. The search is case insensitive.
Oryza sativa ssp japonica Example : WRKY
Search Text
Filter
   Flanking Sequence Tag
   Region  
   Orientation  
Output
   FASTA file
 
 
 
   

Locus Name Search

Locus Name Search allows to retrieve a FSTs with a gene list . Moreover, all the linked FST inside or in the promoter region of the selected genes are displayed.
Oryza sativa ssp. japonica Example : Os01g10900.1
Input
Filter
   Flanking Sequence Tag
   Region  
   Orientation  
Output
   FASTA file
 
 
 
   

Interpro Domain Search

Locus are selected according to one or more Interpro domains. The type of flanking insertion elements, the gene region of insertion and the gene orientation can be used to restrict the search.
Oryza sativa ssp. japonica Example : IPR000009
Entry an Interpro domain
Filter
   Flanking Sequence Tag
   Region  
   Orientation  
Output
   FASTA file
 
 
 
   

BLAST Search

FSTs search in a specific gene can also be done by blasting the Oryza sativa or Arabidopsis thaliana genome with a query sequence. You can cut and paste, or type a sequence into the large text window, or upload a file containing a valid nucleotide or protein sequence in fasta format, from your computer.
Oryza sativa ssp. japonica
Input
Filter
   Database
   Program
   Expect
   Number of alignment
   Flanking Sequence Tag
   Region  
   Orientation  
Output
   FASTA file
 
 
 
   

Primer Blaster

Primer Blaster can be used thereafter to check primers specificity and visualize the targeted region that will be amplified.
Oryza sativa ssp japonica
Cut & Paste sequence Forward   Reverse
 
Or upload file  
Database
Amplicon size Between  and  bp
Mismatch number accepted
   

Primer Designer

Primer Designer is an automatic tool developed to generate primers for FST validation. Users can choice FST, length of amplicon but also to exclude any restriction enzyme in the amplicon. PrimerDesigner help users to design a probe for southern in order to check if a T-DNA is really present or not in the ordered line.
Oryza sativa
Search parameters
  Entry FST or plant name   Ex : AOMA10
 
   bp before FST  -  bp after FST
Restriction Enzyme
Primer3 parameters
  Product size range
  Primer Size Min.  Opt.  Max.  
  Primer TM Min.  Opt.  Max.  
   

Get Sequence

This tool allow to select any genomic sequence by choosing a chromosome number and two coordinates (relative to the top of the chromosome), and display all the genes predicted in the selected region.
Oryza sativa ssp. japonica
Input  
  Chromosome
  From
  To
Output  
 
     -   Start codon   + 
     -   Stop codon   + 
 
 
 
Filter