Keywords Search

Example : WRKY
Search Text
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
   

Locus Search

Example : Os01g10900.1
Input
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
   

Interpro Domain Search

Example : IPR000009
Entry an Interpro domain
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
   

Gene Ontology Search

Example : histidine
Entry an Gene Ontology term
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
   

Primer Designer

Input
Entry a location on a chromosome
For example : Os01:21841..25971

General Primer Picking Conditions
Primer GC% Min.   Opt.   Max. Pair Max Mispriming:
Primer Size Min.   Opt.   Max. Max 3' Stability:
Primer Tm Min.   Opt.   Max. Max Poly-X:
Primer sets: Max Self Complementarity:
Product size range: Max 3' Self Complementarity:
  

Primer Blaster

Cut & Paste sequence Forward   Reverse
 
Or upload file  
Database
Amplicon size Between  and  bp
Mismatch number accepted
   

Get Sequences

Example : Os01:1..20000
Input Location
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
 
 
 
 
   

Genotyping Primer Designer

This program was developed to help users make primers specific to a PCR template. Its combines Primer3 and a specificity check via a BLAST search against Oryza sativa genome.
You just have to cut and paste your FST or plant ID and specify primer parameter.

Input :

General Primer Picking Conditions :
Primer GC% Min.   Opt.   Max. Pair Max Mispriming:
Primer Size Min.   Opt.   Max. Max 3' Stability:
Primer Tm Min.   Opt.   Max. Max Poly-X:
Primer sets: Max Self Complementarity:
Product size range: Max 3' Self Complementarity:
  

Locus Search

Example : BGIOSGA000004
Input
Output
FASTA file
 
   

Interpro Domain Search

Example : IPR000009
Entry an Interpro domain
Output
FASTA file
 
   

Primer Designer

Input
Entry a location on a chromosome
For example : Osi01:264748..269158

General Primer Picking Conditions
Primer GC% Min.   Opt.   Max. Pair Max Mispriming:
Primer Size Min.   Opt.   Max. Max 3' Stability:
Primer Tm Min.   Opt.   Max. Max Poly-X:
Primer sets: Max Self Complementarity:
Product size range: Max 3' Self Complementarity:
  

Primer Blaster

Cut & Paste sequence Forward   Reverse
 
Or upload file  
Database
Amplicon size Between  and  bp
Mismatch number accepted
   

Get Sequences

Example : Osi01:1..20000
Input Location
Output
FASTA file
 
   

Keywords Search

Example : WRKY
Search Text
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
 
 
   

Locus Search

Example : AT1G20900.1
Input
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
 
 
   

Interpro Domain Search

Example : IPR000009
Entry an Interpro domain
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
 
 
   

Primer Designer

Input
Entry a location on a chromosome
For example : At01:153492..157011

General Primer Picking Conditions
Primer GC% Min.   Opt.   Max. Pair Max Mispriming:
Primer Size Min.   Opt.   Max. Max 3' Stability:
Primer Tm Min.   Opt.   Max. Max Poly-X:
Primer sets: Max Self Complementarity:
Product size range: Max 3' Self Complementarity:
  

Primer Blaster

Cut & Paste sequence Forward   Reverse
 
Or upload file  
Database
Amplicon size Between  and  bp
Mismatch number accepted
   

Get Sequences

Example : At01:1..20000
Input Location
Filter
Flanking Sequence Tag
Region  
Orientation  
Output
FASTA file
 
 
 
 
   

Keywords Search

Example : WRKY
Search Text
Output
FASTA file
 
 
   

Locus Search

Example : Sb10g025590.1
Input
Output
FASTA file
 
 
   

Interpro Domain Search

Example : IPR000009
Entry an Interpro domain
Output
FASTA file
 
 
   

Primer Designer

Input
Entry a location on a chromosome
For example : Sb01:161795..168954

General Primer Picking Conditions
Primer GC% Min.   Opt.   Max. Pair Max Mispriming:
Primer Size Min.   Opt.   Max. Max 3' Stability:
Primer Tm Min.   Opt.   Max. Max Poly-X:
Primer sets: Max Self Complementarity:
Product size range: Max 3' Self Complementarity:
  

Primer Blaster

Cut & Paste sequence Forward   Reverse
 
Or upload file  
Database
Amplicon size Between  and  bp
Mismatch number accepted
   

Get Sequences

Example : Sb01:1..20000
Input Location
Output
FASTA file
 
 
   

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